DNA

Part:BBa_K2961006:Design

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-16)


Template DNA sequence coding for HBB rs334 mutation (A to T) in humans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


Design Notes

This part was generated from a CHOPCHOP search and BLAST search that allowed us to find a suitable PAM site containing region of the HBB gene where the rs334 mutation also lies nearby, so that the gRNA we tried to target towards it would be specific to the mutant variant and not the Wildtype variant. There was no suitable perfect PAM site, so it was incorporated by modifying one nucleotide in the genome sequence from a G to an A.


Source

This sequence comes from the human genome sequence.

References