DNA
Part:BBa_K2961006:Design
Designed by: Muhammad Nahin Khan Group: iGEM19_CMUQ (2019-10-16)
Template DNA sequence coding for HBB rs334 mutation (A to T) in humans
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 174
Design Notes
This part was generated from a CHOPCHOP search and BLAST search that allowed us to find a suitable PAM site containing region of the HBB gene where the rs334 mutation also lies nearby, so that the gRNA we tried to target towards it would be specific to the mutant variant and not the Wildtype variant. There was no suitable perfect PAM site, so it was incorporated by modifying one nucleotide in the genome sequence from a G to an A.
Source
This sequence comes from the human genome sequence.